• Catalog Number:

  E1A1000A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15796

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against

• Specificity Sensitivity:

  Smad2 (Phospho-Thr220) antibody detects endogenous levels of Smad2 only when phosphorylated at threonine 220.

• References:

  Riggins G.J., Nat. Genet. 13:347-349(1996). Zhang Y., Nature 383:168-172(1996). Eppert K., Cell 86:543-552(1996).

• All Sites:

  Human: Thr220; Mouse: Thr220; Rat: Thr220

P-peptide - + Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) antibody.