• Catalog Number:

  E1A0029A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15797

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  BSP-1, BSP1, Dwarfin-A, Dwf-A, JV4-1, MADH1, MADR1, Mad-related protein 1, Mad1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, Mothers-against-DPP-related-1, SMAD 1, Smad 1, Transforming growth factor- beta signaling protein-1

• Specificity Sensitivity:

  Smad1 (Phospho-Ser465) antibody detects endogenous levels of Smad1 only when phosphorylated at serine 465.

• References:

  Tino D. Piscione, Am J Physiol Renal Physiol, Jan 2001; 280: 19. Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552. Joan Seoane, Carcinogenesis, Nov 2006; 27: 2148 - 2156.

• All Sites:

  Human: Ser465; Mouse: Ser465; Rat: Ser468

P-peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad1 (phospho-Ser465) antibody. Western blot analysis of extracts from HeLa cells treated with Serum (10%, 15min), using Smad1 (phospho-Ser465) antibody.