• Catalog Number:

  E1A0026A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SHP-1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P29350

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:10000

• Alter Name:

  70Z-SHP, EC 3.1.3.48, HCP, HCPH, Hematopoietic cell protein-tyrosine phosphatase, PTN6, PTP-1C, PTP1C, PTPN6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, Protein-tyrosine phosphatase, non-receptor type 6, SH-PTP1

• Specificity Sensitivity:

  SHP-1 (phospho-Tyr536) antibody detects endogenous levels of SHP-1 only when phosphorylated at tyrosine 536.

• References:

  Laura A. Maile, Mol. Biol. Cell, Sep 2003; 14: 3519. Tony Avril, J. Immunol., Dec 2004; 173: 6841 - 6849. Hong Xu, J. Biol. Chem., Jul 1998; 273: 17987. Gregory Bix, Blood, Dec 2006; 10: 1182.

• All Sites:

  Human: Tyr536; Mouse: Tyr536

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-1 (phospho-Tyr536) antibody.

Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5mins), using SHP-1 (phospho-Tyr536) antibody.