• Catalog Number:

  E1A0025A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Shc around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P29353

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:10000

• Alter Name:

  SH2 domain protein C1, SHC, SHC-transforming protein 1, SHCA, Src homology 2 domain-containing-transforming protein C1

• Specificity Sensitivity:

  Shc (phospho-Tyr427) antibody detects endogenous levels of Shc only when phosphorylated at serine 24.

• References:

  Kerstin Wagner, Carcinogenesis, 2004 25(12):2473-2478. William A. Ricketts, Mol. Endocrinol., Dec 1999; 13: 1988. Aimee W. Kao, Endocrinology, Jun 1997; 138: 2474. Lyn Oligino J. Biol. Chem., Nov 1997; 272: 29046.

• All Sites:

  Human: Tyr427

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using Shc (phospho-Tyr427) antibody.

Western blot analysis of extracts from Hela cells treated with Calyculin A (50ng/ml, 15mins), using Shc (phospho-Tyr427) antibody.