• Catalog Number:

  E1A0020A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 62 (P-L-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P01106

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  Myc proto-oncogene protein, c-myc

• Specificity Sensitivity:

  Myc (phospho-Ser62) antibody detects endogenous levels of Myc only when phosphorylated at serine 62.

• References:

  Markus Welcker, PNAS, Jun 2004; 101: 9085 - 9090. Mark A. Gregory, J. Biol. Chem., Dec 2003; 278: 51606 - 51612. David W. Chang, Mol. Cell. Biol., Jun 2000; 20: 4309 - 4319. Rosalie Sears, Genes & Dev., Oct 2000; 14: 2501.

• All Sites:

  Human: Ser62; Mouse: Ser62; Rat: Ser62

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho-Ser62) antibody.

Western blot analysis of extracts from 293 cells treated with Forskolin (40nM, 30mins), using Myc (phospho-Ser62) antibody.

Forskolin + - Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 15mins), using Myc (phospho-Ser62) antibody.