Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49137
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
EC 2.7.11.1, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPK2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, RPS6KC1, kinase MAPKAPK2
Specificity Sensitivity:
MAPKAPK2 (Phospho-Thr334) antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at threonine 334.
References:
Symone M. San Miguel, J. Biol. Chem., Nov 2005; 280: 37495 - 37502.
Li Xu and Raymond Bergan, AACR Meeting Abstracts, Apr 2006; 2006: 823 - 824.
Atsushi Nakano, Circ. Res., Feb 2000; 86: 144.
All Sites:
Human: Thr334; Mouse: Thr320; Rat: Thr320
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Phospho-Thr334) antibody.
Western blot analysis of extracts from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) antibody.