• Catalog Number:

  E1A0017A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P05787

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:40000

• Alter Name:

  CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8

• Specificity Sensitivity:

  Keratin 8 (phospho-Ser73) antibody detects endogenous levels of Keratin 8 only when phosphorylated at serine 73.

• References:

  Nam-On Ku J. Biol. Chem., Mar 2002; 277: 10775 - 10782. Karen M. Ridge J. Biol. Chem., Aug 2005; 280: 30400 - 30405. Nam-On Ku J. Biol. Chem., Mar 1997; 272: 7556. Gary K. Scott Mol. Pharmacol., Sep 2005; 68: 606 - 615.

• All Sites:

  Human: Ser73; Mouse: Ser79

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (phospho-Ser73) antibody.

Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins), using Keratin 8 (phospho-Ser73) antibody.