• Catalog Number:

  E1A0015A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IκB-ε around the phosphorylation site of serine 22 (I-E-SP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00221

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon, NFKBIE

• Specificity Sensitivity:

  IκB-ε (phospho-Ser22) antibody detects endogenous levels of IκB-ε only when phosphorylated at serine 22.

• References:

  David C. Hay Mol, Cell. Biol., May 2001; 21: 3482 - 3490. Fumitaka Fujita Mol. Cell. Biol., Nov 2003; 23: 7780 - 7793. Dan Tapalaga J. Histochem. Cytochem., Dec 2002; 50: 1599.

• All Sites:

  Human: Ser22; Mouse: Ser22; Rat: Ser22

P-peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IκB-ε (phospho-Ser22) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF-a (20ng/ml, 30mins), using IκB-ε (phospho-Ser22) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using IκB-ε (phospho-Ser22) antibody.