• Catalog Number:

  E1A0013A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08238

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2, HSPCB, Heat shock protein HSP 90-beta

• Specificity Sensitivity:

  HSP90B (phospho-Ser254) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 254.

• References:

  Lothar Goretzki, AACR Meeting Abstracts, Apr 2006; 2006: 468. Jean-François Louvion, Mol. Biol. Cell, Nov 1998; 9: 3071. Angelika Lueking, Mol. Cell. Proteomics, Dec 2003; 2: 1342. Jason Fontana Circ, Res., May 2002; 90: 866.

• All Sites:

  Human: Ser254; Mouse: Ser254; Rat: Ser254

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (phospho-Ser254) antibody.

Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody.

TNF-a + - Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).