• Catalog Number:

  E1A0012A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GSK3α/β around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49840

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  EC 2.7.11.26, Factor A, GSK-3 alpha, GSK3-alpha, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha, GSK-3 beta, GSK3B, Glycogen synthase kinase-3 beta, Protein kinase GSK-3-beta, kinase GSK3-beta

• Specificity Sensitivity:

  GSK3α/β (phospho-Tyr279/216) antibody detects endogenous levels of GSK3α/β only when phosphorylated at tyrosine 279/216.

• References:

  Minsub Shim, J. Biol. Chem., May 2003; 278: 19674 - 19681. Junichi Gotoh Carcinogenesis, Mar 2003; 24: 435 - 442. Katrina MacAulay, J. Biol. Chem., Mar 2005; 280: 9509 - 9518. Victor H. Bustos, PNAS, Dec 2006; 103: 19725 - 19730.

• All Sites:

  Human: Tyr279/216; Mouse: Tyr279/216; Rat: Tyr279/216

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK3α/β (phospho-Tyr279/216) antibody.

Western blot analysis of extracts from HeLa cells, using GSK3α/β (phospho-Tyr279/216) antibody.