• Catalog Number:

  E1A0985A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-TP-Q-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P15927

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  REPA2, RF-A, RP-A, RPA2, RPA32, replication factor-A protein 2, replication protein A 32 kDa subunit, replication protein A2

• Specificity Sensitivity:

  RFA2 (Phospho-Thr21) antibody detects endogenous levels of RFA2 only when phosphorylated at threonine 21.

• References:

  Erdile L.F., J. Biol. Chem. 265:3177-3182(1990). Gregory S.G., Nature 441:315-321(2006). Niu H., J. Biol. Chem. 272:12634-12641(1997). Cho J.M., Nucleic Acids Res. 28:3478-3485(2000).

• All Sites:

  Human: Thr21; Mouse: Thr21

Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.