• Catalog Number:

  E1A0984A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of threonine 826 (K-M-TP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein

• Specificity Sensitivity:

  Retinoblastoma (Phospho-Thr826) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at threonine 826.

• References:

  Lee W.-H., Nature 329:642-645(1987). Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987). McGee T.L., Gene 80:119-128(1989).

• All Sites:

  Human: Thr826; Mouse: Thr817; Rat: Thr797

P-peptide - + Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using Retinoblastoma (Phospho-Thr826) antibody. Immunofluorescence analysis of COS7 cells, using Retinoblastoma (Phospho-Thr826) antibody.