• Catalog Number:

  E1A0982A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 249 (N-G-SP-P-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein

• Specificity Sensitivity:

  Retinoblastoma (Phospho-Ser249) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 249.

• References:

  Ishizaka Y., Oncogene 4:789-794(1989). Angrist M., Hum. Mol. Genet. 4:821-830(1995). Sjoeblom T., Science 314:268-274(2006).

• All Sites:

  Human: Ser249; Mouse: Ser243; Rat: Ser221

P-peptide - + Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Ser249) antibody.