• Catalog Number:

  E1A0079A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLCG1 around the phosphorylation site of tyrosine 771 (P-D-YP-G-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19174

• Applications:

  WB: 1: 500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  Phospholipase C gamma 1,PLC148, PHOSPHOLIPASE C-148

• Specificity Sensitivity:

  PLCG1 (phospho-Tyr771) antibody detects endogenous levels of PLCG1 only when phosphorylated at tyrosine 771.

• References:

  Denis Tvorogov, J. Cell Sci., Feb 2005; 118: 601 - 610. Brenda J. Irvin, Mol. Cell. Biol., Dec 2000; 20: 9149 - 9161. Benoit Poulin, J. Biol. Chem., Feb 2000; 275: 6411. Ichiro Nakamura, J. Cell Biol., Jan 2001; 152: 361.

• All Sites:

  Human: Tyr771; Mouse: Tyr771; Rat: Tyr771

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLCG1 (phospho-Tyr771) antibody.

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using PLCG1 (phospho-Tyr771) antibody.