Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PLC-β around the phosphorylation site of serine 1105 (N-N-SP-I-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PLC-β (phospho-Ser1105) antibody detects endogenous levels of PLC-β only when phosphorylated at serine 1105.
References:
Chunzhi Xia, J. Biol. Chem., Jun 2001; 276: 19770 - 19777.
Helena Frecker, Am J Physiol Renal Physiol, Nov 2005; 289: F1078 - F1087.
Clive P. Morgan, J. Biol. Chem., Nov 2004; 279: 47159 - 47171.
All Sites:
Human: Ser1105; Mouse: Ser1105; Rat: Ser1105
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLC-β (phospho-Ser1105) antibody.
Western blot analysis of extracts from A431 cells, using PLC-β (phospho-Ser1105) antibody.