• Catalog Number:

  E1A0077A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 410 (G-V-YP-A-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P56945

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  BCAR1, Breast cancer anti-estrogen resistance 1, CRK-ASSOCIATED SUBSTRATE, CRKAS

• Specificity Sensitivity:

  p130 Cas (Phospho-Tyr410) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 410.

• References:

  Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535. Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863. Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189. Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6858.

• All Sites:

  Human: Tyr410; Mouse: Tyr414; Rat: Tyr508

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (#A0077).

Western blot analysis of extracts from NIH/3T3 cells, using p130 Cas (Phospho-Tyr410) antibody (#A0077).