• Catalog Number:

  E1A0075A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human KSR around the phosphorylation site of serine 392 (T-E-SP-V-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8IVT5

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  CAP kinase, HB, HB protein, Kinase suppressor of RAS-1, ceramide-activated protein kinase, kinase KSR1

• Specificity Sensitivity:

  KSR (phospho-Ser392) antibody detects endogenous levels of KSR only when phosphorylated at serine 392.

• References:

  Angela M. Cacace, Mol. Cell. Biol., Jan 1999; 19: 229 - 240. François Roy, Genes & Dev., Feb 2002; 16: 427. DK Morrison, J. Cell Sci., May 2001; 114: 1609. Christine E. Horak, AACR Meeting Abstracts, Mar 2004; 2004: 564

• All Sites:

  Human: Ser164; Mouse: Ser392

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using KSR (phospho-Ser392) antibody.

Western blot analysis of extracts from HepG2 cells, using KSR (phospho-Ser392) antibody.