• Catalog Number:

  E1A0074A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSL around the phosphorylation site of serine 855/554 (S-V-SP-E-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05469

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  EC 3.1.1.79, Hormone sensitive lipase, LIPE, LIPS

• Specificity Sensitivity:

  HSL (Phospho-Ser855/554) antibody detects endogenous levels of HSL only when phosphorylated at serine 855/554.

• References:

  Matthew J. Watt, Am J Physiol Endocrinol Metab, Mar 2006; 290: E500 - E508. Maya S. Gowri, Am J Physiol Endocrinol Metab, Sep 2000; 279: E593 - E600. Matthew J. Watt, Am J Physiol Endocrinol Metab, Jul 2004; 287: E120 - E127. Shu Pei Wang, Endocrinology, Dec 2004; 145: 5688 - 5693.

• All Sites:

  Human: Ser855; Mouse: Ser559; Rat: Ser865

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using HSL (Phospho-Ser855/554) antibody (#A0074).

Western blot analysis of extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), using HSL (Phospho-Ser855/554) antibody (#A0074).