• Catalog Number:

  E1A0073A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GR around the phosphorylation site of serine 211 (N-E-SP-P-W).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04150

• Applications:

  WB: 1: 500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  GCR, GRL, GRL1, NR3C1, glucocorticoid receptor

• Specificity Sensitivity:

  GR (phospho-Ser211) antibody detects endogenous levels of GR only when phosphorylated at serine 211.

• References:

  Zhen Wang, Mol. Endocrinol., Mar 2007; 21: 625 - 634. Aaron L. Miller, Mol. Endocrinol., Jun 2005; 19: 1569 - 1583. Paul J. LeBlanc, J. Physiol., Jun 2004; 557: 559 - 570. José Xavier-Neto, Hypertension, Jul 1999; 34: 31.

• All Sites:

  Human: Ser211; Mouse: Ser220; Rat: Ser232

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GR (phospho-Ser211) antibody.

Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 30min), using GR (phospho-Ser211) antibody.