• Catalog Number:

  E1A0072A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Filamin A around the phosphorylation site of serine 2152 (A-P-SP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P21333

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  ABP-280, Alpha-filamin, Endothelial actin-binding protein, FLN, FLN1, Filamin 1, Nonmuscle filamin, filamin A

• Specificity Sensitivity:

  Filamin A (phospho-Ser2152) antibody detects endogenous levels of Filamin A only when phosphorylated at serine 2152.

• References:

  Atul Deshmukh, Diabetes, Jun 2006; 55: 1776 - 1782. Michele S. Woo, Mol. Cell. Biol., Apr 2004; 24: 3025 - 3035. D. Wallach, J. Biol. Chem., Jul 1978; 253: 4739 - 4745. Kyeong-Man Kim, J. Biol. Chem., Apr 2005; 280: 12774 - 12780.

• All Sites:

  Human: Ser2152; Mouse: Ser2152; Rat: Ser2239

Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 5mins), using Filamin A (phospho-Ser2152) antibody.