• Catalog Number:

  E1A0071A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00418

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  stathmin 1, LAG, LAP18, Leukemia-associated gene protein, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Op18, PR22, Phosphoprotein p19, Pr22 protein, Prosolin, STN1, pp17, pp19, stathmin

• Specificity Sensitivity:

  eEF2K (phospho-Ser366) antibody detects endogenous levels of eEF2K only when phosphorylated at serine 366.

• References:

  Sandrine Horman, J. Biol. Chem., Oct 2003; 278: 41970 - 41976. Ly Q. Hong-Brown, J. Biol. Chem., Feb 2007; 282: 3702 - 3712. Hening Ren, Cancer Res., Jul 2005; 65: 5841 - 5847. Huanchun Zhang, J. Biol. Chem., Apr 2007; 10.1074.

• All Sites:

  Human: Ser366; Mouse: Ser365; Rat: Ser365

Western blot analysis of extracts from Hela cells treated with serum (10%, 15mins), using eEF2K (phospho-Ser366) antibody.