• Catalog Number:

  E1A0070A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eEF2 around the phosphorylation site of threonine 56 (R-F-TP-D-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P13639

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:10000

• Alter Name:

  EEF2, EF-2, EF2, Elongation factor 2

• Specificity Sensitivity:

  eEF2 (Phospho-Thr56) antibody detects endogenous levels of eEF2 only when phosphorylated at threonine 56.

• References:

  David M. Thomson, FASEB J, Mar 2006; 20: A819. Scott E. Gordon, FASEB J, Mar 2006; 20: A819. Xu Li, FEBS J., Aug 2005; 272: 4211 - 4220. Adam J. Rose, J. Physiol., Nov 2005; 569: 223 - 228.

• All Sites:

  Human: Thr56; Mouse: Thr56; Rat: Thr56

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho-Thr56) antibody (#A0070).

Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30mins), using eEF2 (Phospho-Thr56) antibody (#A0070).

P-peptide - + Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) antibody (#A0070).