• Catalog Number:

  E1A0069A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P24864

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  CCNE, CCNE1, G1/S-specific cyclin E1

• Specificity Sensitivity:

  Cyclin E1 (phospho-Thr395) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395.

• References:

  Wentao Deng, J. Virol., Dec 2004; 78: 13954 - 13965. Tianlin Ma, PNAS, Jan 1999; 96: 382. Nathalie Cueille, J. Virol., Sep 1998; 72: 7255 - 7262. Chiung-Yueh Hsu, J. Virol., Jan 2007; 81: 384 - 394.

• All Sites:

  Human: Thr395

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Cyclin E1 (phospho-Thr395) antibody.

Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (phospho-Thr395) antibody.