• Catalog Number:

  E1A0068A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P14635

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1

• Specificity Sensitivity:

  Cyclin B1 (Phospho-Ser126) antibody detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.

• References:

  Jing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609. Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280. Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074. Jianke Li, PNAS, Jan 1997; 94: 502.

• All Sites:

  Human: Ser126; Mouse: Ser123

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin B1 (Phospho-Ser126) antibody (#A0068).

Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 15mins), using Cyclin B1 (Phospho-Ser126) antibody (#A0068).