• Catalog Number:

  E1A0067A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207(H-A-YP-A-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P16949

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Alter Name:

  stathmin 1, LAG, LAP18, Leukemia-associated gene protein, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Op18, PR22, Phosphoprotein p19, Pr22 protein, Prosolin, STN1, pp17, pp19, stathmin

• Specificity Sensitivity:

  CrkL (phospho-Tyr207) antibody detects endogenous levels of CrkL only when phosphorylated at tyrosine 207.

• References:

  Martin Sattler, J. Biol. Chem., Apr 1997; 272: 10248. J ten Hoeve, Blood, Sep 1994; 84: 1731 - 1736. Andrew A. Butler, J. Biol. Chem., Oct 1997; 272: 27660. Franck Gesbert, J. Biol. Chem., Feb 1998; 273: 3986.

• All Sites:

  Human: Tyr207; Mouse: Tyr207

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CrkL (phospho-Tyr207) antibody.

Western blot analysis of extracts from COS7 cells, using CrkL (phospho-Tyr207) antibody.

P-peptide - + Immunofluorescence analysis of HuvEc cells, using CrkL (phospho-Tyr207) antibody.