• Catalog Number:

  E1A0066A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CrkII around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P46108

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  Adapter molecule crk, CRKO, Crk-II, P38, Proto-oncogene C-crk

• Specificity Sensitivity:

  CrkII (phospho-Tyr221) antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine 221.

• References:

  Robert F. Spurney, J. Clin. Invest., May 2002; 109: 1361. Faiyaz Ahmad, J. Immunol., Apr 1999; 162: 4864. Feng Liu, J. Biol. Chem., Dec 1996; 271: 31290. Jaap J. Van Hellemond, J. Biol. Chem., Mar 2000; 275: 8315.

• All Sites:

  Human: Tyr221; Mouse: Tyr221; Rat: Tyr221

P-peptide - + Western blot analysis of extracts from cos7 cells, using CrkII (phospho-Tyr221) antibody. Immunofluorescence analysis of HuvEc cells, using CrkII (phospho-Tyr221) antibody.