• Catalog Number:

  E1A0065A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-PLA2 around the phosphorylation site of serine 505 (P-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P47712

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:10000

• Alter Name:

  CPLA2, Cytosolic phospholipase A2, EC 3.1.1.4, EC 3.1.1.5, PA24, PA24A, PLA2G4, PLA2G4A, Phosphatidylcholine 2-acylhydrolase

• Specificity Sensitivity:

  c-PLA2 (Phospho-Ser505) antibody detects endogenous levels of c-PLA2 only when phosphorylated at serine 505.

• References:

  D. D. Denson, J Neurophysiol, Jun 2005; 93: 3146 - 3156. Diane M. Duffy, J. Clin. Endocrinol. Metab., Oct 2005; 90: 5858 - 5865. Andrei I. Ivanov, Am J Physiol Regulatory Integrative Comp Physiol, Nov 2002; 283: 1104. Miriam Kolko, Invest. Ophthalmol. Vis. Sci., Mar 2007; 48: 1401 - 1409.

• All Sites:

  Human: Ser505; Mouse: Ser505; Rat: Ser505

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using c-PLA2 (Phospho-Ser505) antibody (#A0065).

Western blot analysis of extracts from HeLa cells treated with TNF-α (20ng/ml, 30mins), using c-PLA2 (Phospho-Ser505) antibody (#A0065).

TNF-a + - Immunofluorescence analysis of HeLa cells treated with TNF-α (20nM, 15mins), using c-PLA2 (Phospho-Ser505) antibody (#A0065).