• Catalog Number:

  E1A0064A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P41279

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  EC 1.14.16.2, TH isoform 3, TH isoform a, TH-4, TY3H, TYH, Tyrosine 3-hydroxylase, Tyrosine 5-monooxygenase, tyrosine hydroxylase

• Specificity Sensitivity:

  COT (phospho-Thr290) antibody detects endogenous levels of COT only when phosphorylated at threonine 290.

• References:

  Jahan Ara, PNAS, Jun 1998; 95: 7659. E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107. P. William Conrad, J. Biol. Chem., Nov 1999; 274: 33709. Y. Katayama, J Appl Physiol, Nov 1994; 77: 2086.

• All Sites:

  Human: Thr290; Mouse: Thr290; Rat: Thr290

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (phospho-Thr290) antibody.

Western blot analysis of extracts from 293 cells, treated with UV (15mins), using COT (phospho-Thr290) antibody.

P-peptide - + Immunofluorescence analysis of HuvEc cells, using COT (phospho-Thr290) antibody (#11364).