• Catalog Number:

  E1A0063A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 323 (S-P-SP-M-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P30305

• Applications:

  WB: 1:500~1000 ELISA: 1:20000

• Alter Name:

  CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2

• Specificity Sensitivity:

  CDC25B (Phospho-Ser323) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.

• References:

  Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531. Véronique Baldin, J. Biol. Chem., Dec 1997; 272: 32731. Estelle Schmitt, J. Cell Sci., Oct 2006; 119: 4269 - 4275.

• All Sites:

  Human: Ser323; Mouse: Ser321; Rat: Ser319

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody (#A0063).