Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CCR5 around the phosphorylation site of serine 336 (R-A-SP-S-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
CCR5 (Phospho-Ser336) antibody detects endogenous levels of CCR5 only when phosphorylated at serine 336.
References:
Martin Oppermann, J. Biol. Chem., Mar 1999; 274: 8875.
Beatrix Pollok-Kopp, J. Biol. Chem., Jan 2003; 278: 2190 - 2198.
Ramtin Rahbar, J. Virol., Jul 2006; 80: 7245 - 7259.
All Sites:
Human: Ser336; Mouse: Ser338; Rat: Ser338
Western blot analysis of extracts from Jurkat cells, using CCR5 (Phospho-Ser336) antibody.