• Catalog Number:

  E1A0060A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  P55211

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  APAF-3; apoptotic protease activating factor 3; apoptotic protease Mch-6; CASP-9; caspase-9; caspase9; EC 3.4.22.62; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; RNCASP9

• Specificity Sensitivity:

  Caspase 9 (Phospho-Thr125) antibody detects endogenous levels of Caspase 9 only when phosphorylated at threonine 125.

• References:

  Duan H., J. Biol. Chem. 271:16720-16724(1996). Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996). Hadano S., Mamm. Genome 10:757-760(1999).

• All Sites:

  Human: Thr125; Mouse: Thr163; Rat: Thr163

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 5mins) and calyculinA (50ng/ml, 15mins), using Caspase 9 (Phospho-Thr125) antibody.