• Catalog Number:

  E1A0059A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 8 around the phosphorylation site of serine 347 (C-P-SP-L-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14790

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 precursor, EC 3.4.22.-, FADD-homologous ICE/CED-3- like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE8, MACH, MCH5, MORT1-associated CED-3 homolog

• Specificity Sensitivity:

  Caspase 8 (Phospho-Ser347) antibody detects endogenous levels of Caspase 8 only when phosphorylated at serine 347.

• References:

  Daniel R. Beisner, J. Immunol., Sep 2005; 175: 3469 - 3473. Peter J. Wild, Mol. Cancer Ther., Apr 2005; 4: 516 - 528. A.V. TIMOFEEVA, Ann. N.Y. Acad. Sci., Dec 2006; 1091: 319 - 335.

• All Sites:

  Human: Ser347; Mouse: Ser349

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Caspase 8 (Phospho-Ser347) antibody. Western blot analysis of extracts from Jurkat cells, using Caspase 8 (Phospho-Ser347) antibody.