• Catalog Number:

  E1A0057A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C/EBP-β around the phosphorylation site of threonine 235/188 (P-G-TP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17676

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Alter Name:

  AGP/EBP, C/EBP beta, C/EBP-related protein 2, CCAAT/enhancer binding protein beta, CEBPB, CRP2, IL-6DBP, Interleukin-6- dependent binding protein, LAP, Liver-enriched transcriptional activator, Nuclear factor NF-IL6, SF-B, SFB, Silencer factor B, TCF5, Transcription factor 5

• Specificity Sensitivity:

  C/EBP-β (phospho-Thr235/188) antibody detects endogenous levels of C/EBP-β only when phosphorylated at threonine 235/188.

• References:

  Songyun Zhu, PNAS, Jan 2002; 99: 207. Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180. Béatrice Thomas, Eur. J. Biochem., Dec 2000; 267: 6798. Edoardo Parrella, J. Biol. Chem., Oct 2004; 279: 42026 - 42040.

• All Sites:

  Human: Thr235; Mouse: Thr188; Rat: Thr189

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using C/EBP-β (phospho-Thr235/188) antibody.

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using C/EBP-β (phospho-Thr235/188) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using C/EBP-β (phospho-Thr235/188) antibody (#11357).