• Catalog Number:

  E1A0056A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C/EBP-α around the phosphorylation site of serine 21 (L-Q-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49715

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 precursor, EC 3.4.22.-, FADD-homologous ICE/CED-3- like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE8, MACH, MCH5, MORT1-associated CED-3 homolog

• Specificity Sensitivity:

  C/EBP-α (phospho-Ser21) antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.

• References:

  Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461. Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204. Raphaël Métivier, Mol. Endocrinol., Nov 2001; 15: 1953. Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

• All Sites:

  Human: Ser21; Mouse: Ser21; Rat: Ser21

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody.

Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 5mins), using C/EBP-α (phospho-Ser21) antibody.