• Catalog Number:

  E1A0054A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 223 (A-L-YP-D-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q06187

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  AGMX1, ATK, Agammaglobulinaemia tyrosine kinase, B cell progenitor kinase, BPK, Bruton's tyrosine kinase, EC 2.7.10.2, Kinase EMB, kinase Btk

• Specificity Sensitivity:

  BTK (phospho-Tyr223) antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.

• References:

  Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864. Hiroo Ueno, Mol. Biol. Cell, Feb 2000; 11: 735. Hidenori Hase, J. Biol. Chem., Nov 2002; 277: 46950 - 46958.

• All Sites:

  Human: Tyr223; Mouse: Tyr223; Rat: Tyr224

Western blot analysis of extracts from Hela cells treated with serum (10%, 15mins), using BTK (phospho-Tyr223) antibody.