• Catalog Number:

  E1A0053A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BIK around the phosphorylation site of threonine 33 (G-M-TP-D-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q13323

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  Apoptosis inducer NBK, BIKLK, BIP1, BP4, Bcl-2 interacting killer, NBK

• Specificity Sensitivity:

  BIK (Phospho-Thr33) antibody detects endogenous levels of BIK only when phosphorylated at threonine 33.

• References:

  Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676. Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633. Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449. Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.

• All Sites:

  Human:Thr33

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using BIK (Phospho-Thr33) antibody (#A0053).

Western blot analysis of extracts from HepG2 cells, using BIK (Phospho-Thr33) antibody (#A0053, Line 1 and 2).