• Catalog Number:

  E1A0051A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human acetyl-CoA carboxylase around the phosphorylation site of serine 80 (S-M-SP-G-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13085

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  ACAC, ACACA, ACC-alpha, ACCA, Acetyl-CoA carboxylase 1, EC 6.3.4.14, EC 6.4.1.2

• Specificity Sensitivity:

  Acetyl-CoA Carboxylase (phospho-Ser80) antibody detects endogenous levels of acetyl-CoA carboxylase only when phosphorylated at serine 80.

• References:

  JB Allred, J. Lipid Res., Apr 1983; 24: 449. TL Swenson and JW Porter, J. Biol. Chem., Mar 1985; 260: 3791 - 3797. B. A. Lent, J. Biol. Chem., Nov 1978; 253: 8149 - 8156. W. W. Winder, J Appl Physiol, Jan 1997; 82: 219.

• All Sites:

  Human: Ser80; Mouse: Ser79; Rat: Ser79

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Acetyl-CoA Carboxylase (phospho-Ser80) antibody (#A0051).

Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 5mins), using Acetyl-CoA Carboxylase (phospho-Ser80) antibody (#A0051, Line 1 and 2).