• Catalog Number:

  E1A0039A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tyrosine Hydroxylase around the phosphorylation site of serine 40 (R-Q-SP-L-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q2M3B4

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:40000

• Alter Name:

  EC 1.14.16.2, TH isoform 3, TH isoform a, TH-4, TY3H, TYH, Tyrosine 3-hydroxylase, Tyrosine 5-monooxygenase, tyrosine hydroxylase

• Specificity Sensitivity:

  Tyrosine Hydroxylase (phospho-Ser40) antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at serine 40.

• References:

  Jahan Ara, PNAS, Jun 1998; 95: 7659. E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107. P. William Conrad, J. Biol. Chem., Nov 1999; 274: 33709. Y. Katayama, J Appl Physiol, Nov 1994; 77: 2086.

• All Sites:

  Human: Ser71; Mouse: Ser40; Rat: Ser40

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using Tyrosine Hydroxylase (phospho-Ser40) antibody.

Western blot analysis of extracts from RAW264.7 cells treated with UV (30mins), using Tyrosine Hydroxylase (phospho-Ser40) antibody.