• Catalog Number:

  E1A0035A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Trk B around the phosphorylation site of tyrosine 515 (P-Q-YP-F-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16620

• Applications:

  WB: 1:500~3000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  BDNF/NT-3 growth factors receptor precursor, EC 2.7.10.1, GP145-TrkB, GP145-TrkB/GP95-TrkB, NTRK2, Trk-B, TrkB tyrosine kinase, kinase TrkB

• Specificity Sensitivity:

  Trk B (phospho-Tyr515) antibody detects endogenous levels of Trk B only when phosphorylated at tyrosine 515.

• References:

  Tina Garofalo, J. Biol. Chem., Dec 1998; 273: 35153. VI Shifrin and BG Neel, J. Biol. Chem., Dec 1993; 268: 25376 - 25384. Jan Grimm, J. Cell Biol., Jul 2001; 154: 345. Jose L. Tomsig and Terry T. Turner, J. Androl, May 2006; 27: 348 - 357.

• All Sites:

  Human: Tyr516; Mouse: Tyr515; Rat: Tyr515

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue, using Trk B (phospho-Tyr515) antibody.

Western blot analysis of extracts from NIH/3T3 cells, using Trk B (phospho-Tyr515) antibody.