• Catalog Number:

  E1A0032A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309 (G-L-SP-D-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P21579

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  SYT, p65, synaptotagmin I

• Specificity Sensitivity:

  Synaptotagmin (phospho-Ser309) antibody detects endogenous levels of Synaptotagmin only when phosphorylated at serine 309.

• References:

  E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107. Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143. Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909. Vladimir I. Slepnev, Science, Aug 1998; 281: 821.

• All Sites:

  Human: Ser309; Mouse: Ser308

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Ser309) antibody.

Western blot analysis of extracts from 293 cells treated with Sobital (0.4M, 30mins), using Synaptotagmin (phospho-Ser309) antibody.