• Catalog Number:

  E1A0010A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ephrin B around the phosphorylation site of tyrosine 330 (N-I-YP-Y-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P52799

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  CEK5 ligand, CEK5 receptor ligand, CEK5-L, CEL5-L, EFL-3, EFNB1, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPL2, EPLG2, Ephrin-B1 precursor, LERK-2, LERK2, STRA1, STRA1 protein, kinase ephrin-B1

• Specificity Sensitivity:

  Ephrin B (phospho-Tyr330) antibody detects endogenous levels of Ephrin B only when phosphorylated at tyrosine 330.

• References:

  Agnieszka Stokowski Stem, Cells, Jan 2007; 25: 156 - 164. Hans-Christian Aasheim, Blood, Apr 2005; 105: 2869 - 2876. Zhengding Su Eur, J. Biochem., May 2004; 271: 1725 - 1736. Zhen Xu, J. Biol. Chem., Jun 2003; 278: 24767 - 24775.

• All Sites:

  Human: Tyr330; Mouse: Tyr333; Rat: Tyr342

Western blot analysis of extracts from 293 cells treated with TNF-α (20ng/ml, 30mins), using Ephrin B (phospho-Tyr330) antibody.