• Catalog Number:

  E1A0007A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UD71

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B

• Specificity Sensitivity:

  DARPP-32 (phospho-Thr75) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.

• References:

  JA Girault, J. Biol. Chem., Dec 1989; 264: 21748 - 21759. GL Snyder, J. Neurosci., Aug 1992; 12: 3071. Akinori Nishi, J. Neurosci., Nov 1997; 17: 8147. Artur Mayerhofer, J. Clin. Endocrinol. Metab., Dec 2000; 85: 4750 - 4757.

• All Sites:

  Human:Thr75; Mouse: Thr75; Rat: Thr75

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using DARPP-32 (phospho-Thr75) antibody.

Western blot analysis of extracts from COS7 cells treated with Forskolin (40nM, 30mins), using DARPP-32 (phospho-Thr75) antibody.