Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CaMKII around the phosphorylation site of threonine 305 (I-L-TP-T-M).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q9UQM7
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
CAMK2A, CaM-kinase II alpha chain, CaMK-II alpha subunit, CaMKII-alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, EC 2.7.11.17, KCC2A, kinase CaMK2-alpha
Specificity Sensitivity:
CaMKII (Phospho-Thr305) antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 305.
References:
Carl W. Tong, J. Physiol., Aug 2004; 558: 927 - 941.
Pierre R, J. Biol. Chem., Sep 1997; 272: 24133.
Daliang Wang, PNAS, Jun 1998; 95: 7133.
All Sites:
Human: Thr305; Mouse: Thr305; Rat: Thr305
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using CaMKII (Phospho-Thr305) Antibody.
Western blot analysis of extracts from NIH/3T3 cells, using CaMKII (Phospho-Thr305) Antibody.