• Catalog Number:

  E1A0003A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPKα around the phosphorylation site of threonine172 (L-R-TP-S-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13131

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, EC 2.7.11.1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1

• Specificity Sensitivity:

  AMPKα (Phospho-Thr172) antibody detects endogenous levels of AMPKα only when phosphorylated at threonine172 in both chain 1 and 2.

• References:

  Siwang Yu AACR, Meeting Abstracts, Apr 2006; 2006: 538. Laura BarréAm, J Physiol Endocrinol Metab, Mar 2007; 292: E802 - E811. Hideyuki Sakoda, Am J Physiol Endocrinol Metab, Sep 2005; 289: E474 - E481. Lucie Tosca Endocrinology, Oct 2005; 146: 4500 - 4513.

• All Sites:

  Human: Thr172; Mouse: Thr172; Rat: Thr172

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα (Phospho-Thr172) antibody.

Western blot analysis of extracts from 293 cells treated with heat shock, using AMPKα (Phospho-Thr172) antibody.