• Catalog Number:

  E1A0002A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ADD1around the phosphorylation site of serine 726 (T-P-SP-F-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35611

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:40000

• Alter Name:

  ADDA, Alpha adducin, Erythrocyte adducin alpha subunit

• Specificity Sensitivity:

  ADD1 (phospho-Ser726) antibody detects endogenous levels of ADD1 only when phosphorylated at serine 726.

• References:

  Yun Sok Lee, Mol. Cell. Biol., Jan 2007; 27: 438 - 452. Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006. Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768. Jae Bum Kim, J. Clin. Invest., Jan 1998; 101: 1.

• All Sites:

  Human: Ser726; Mouse: Ser724; Rat: Ser724

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 (phospho-Ser726) antibody (#A0002).

Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30mins), using ADD1 (phospho-Ser726) antibody (#A0002).

P-peptide - + Immunofluorescence analysis of HeLa cells, using ADD1 (phospho-Ser726) antibody (#A0002).