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Catalog Number:
E12-006-1 Amount:
30ul Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability:
Store at -20℃/1 year Applications:
WB: 1:5,000 IP: 1:200 IF: 1:1,000 Specificity Sensitivity:
The VSV-G tag antibody can recognize C-terminal, internal, and N-terminal VSV-G fusion proteins.
1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 (lane 1)and 1:10,000 (lane 2).
IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with VSV-G-tag fusion protein
3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G).