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VSV-G-Tag Mouse Monoclonal Antibody
TRANSFEC
MMA
Catalog Number: E12-006-1
Amount: 30ul
Applications:
WB
IF
IP
Reactivity:
产品类型: Unconjugated
发货周期: 现货
说明书:     PDF
总价格:    430元
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概述
  • Catalog Number:

    E12-006-1
  • Amount:

    30ul
  • Form of Antibody:

    Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage/Stability:

    Store at -20℃/1 year
  • Applications:

    WB: 1:5,000 IP: 1:200 IF: 1:1,000
  • Specificity Sensitivity:

    The VSV-G tag antibody can recognize C-terminal, internal, and N-terminal VSV-G fusion proteins.


1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 (lane 1)and 1:10,000 (lane 2).

1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 (lane 1)and 1:10,000 (lane 2).


IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with VSV-G-tag fusion protein
3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G).

IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli) IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G).

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