Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from humanHER2 around the phosphorylation site of threonine 686 (K-Y-TP-M-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
HER2 (Ab-686) antibody detects endogenous levels of total HER2 protein.
References:
Yamamoto T., Nature 319:230-234(1986).
Coussens L., Science 230:1132-1139(1985).
Rieder M.J., Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using HER2 (Ab-686) antibody.
Western blot analysis of extracts from HUVEC cells, using HER2 (Ab-686) antibody.