Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GATA4 around the phosphorylation site of serine 262 (R-L-SP-A-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P43694
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Other Names:
DNA binding protein GATA-GT2, GAT4, GATA binding factor-4, GATA-4, Transcription factor GATA-4
Specificity Sensitivity:
GATA4 (Ab-262) antibody detects endogenous levels of total GATA4 protein.
References:
Audrey Holtzinger. Development, Sep 2005; 132: 4005 - 4014.
José Rivera-Feliciano. Development, Sep 2006; 133: 3607 - 3618.
Elisabeth M. Zeisberg. J. Clin. Invest., Jun 2005; 115: 1522 - 1531.
Jakub Kwintkiewicz. Mol. Endocrinol., Apr 2007; 21: 933 - 947.
Peptide - +
Western blot analysis of extracts from COLO205 cells, treated with Serum (20%, 15mins), using GATA4 (Ab-262) antibody.
Immunofluorescence analysis of HuvEc cells, using GATA4 (Ab-262) antibody.