• Catalog Number:

  E1A0920B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human ERK8 around the phosphorylation site of threonine 175 and tyrosine 177 (A-V-TP-E-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8TD08

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Mitogen-activated protein kinase 15, EC 2.7.11.24, Extracellular signal-regulated kinase 8, MAPK15, ERK8

• Specificity Sensitivity:

  ERK8 (Ab-175/177) antibody detects endogenous levels of total ERK8 protein.

• References:

  Abe M.K., J. Biol. Chem. 277:16733-16743(2002). Iavarone C., J. Biol. Chem. 281:10567-10576(2006). Saelzler M.P., J. Biol. Chem. 281:16821-16832(2006).

Peptide - + Western blot analysis of extracts from HepG2 cells, using ERK8 (Ab-175/177) antibody. Immunofluorescence analysis of NIH/3T3 cells, using ERK8 (Ab-175/177) antibody.