Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Epo-R around the phosphorylation site of tyrosine 368 (D-T-YP-L-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19235
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
Other Names:
EPO-R, EPOR, Erythropoietin receptor precursor
Specificity Sensitivity:
Epo-R (Ab-368) antibody detects endogenous levels of total Epo-R protein.
References:
Jones S.S., Blood 76:31-35(1990).
Noguchi C.T., Blood 78:2548-2556(1991).
Ehrenman K., Exp. Hematol. 19:973-977(1991).
Nakamura Y., Science 257:1138-1141(1992).
Peptide - +
Western blot analysis of extracts from COS7 cells, treated with EPO (20U/ml, 15mins), using Epo-R (Ab-368) antibody.
Immunofluorescence analysis of HeLa cells, using Epo-R (Ab-368) antibody.